anti human ptprn (Proteintech)
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Proteintech
anti human ptprn
Anti Human Ptprn, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+human+ptprn/IA-2%2FPTPRN+Antibody/pmc08207963-12-0-5
Average 92 stars, based on 6 article reviews
Anti Human Ptprn, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+human+ptprn/IA-2%2FPTPRN+Antibody/pmc08207963-12-0-5
Average 92 stars, based on 6 article reviews
anti human ptprn - by Bioz Stars,
2026-09
92/100 stars
Images
1) Product Images from "Overexpression of PTPRN Promotes Metastasis of Lung Adenocarcinoma and Suppresses NK Cell Cytotoxicity"
Article Title: Overexpression of PTPRN Promotes Metastasis of Lung Adenocarcinoma and Suppresses NK Cell Cytotoxicity
Journal: Frontiers in Cell and Developmental Biology
doi: 10.3389/fcell.2021.622018
Figure Legend Snippet: Antibodies used for WB and IHC.
Techniques Used:
Figure Legend Snippet: Association of PTPRN expression with OS by IHC. (A) IHC is used to stain for PTPRN in tumor samples from 40 LUAD patients. Representative IHC pictures of the high and low PTPRN expression groups are shown at ×20 and ×40 magnification (scale bars, 100 μm and 50 μm, respectively). (B) Kaplan–Meier survival analysis of 40 LUAD patients shows that the PTPRN high expression is related to unfavorable prognosis.
Techniques Used: Expressing, Staining
Figure Legend Snippet: Effect of overexpression of PTPRN expression on migration of LUAD cells. (A) The expression of PTPRN in A549 and H1299 cells after transfection of the PTPRN plasmid. (B) The effect on migration of A549- and H1299-overexpressed PTPRN is detected by wound healing assay. (C) The effect on migration of A549- and H1299-overexpressed PTPRN is identified by transwell migration assay. Original magnification, ×100. Scale bars, 100 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. oe-NC, negative control; and oe-PTPRN, PTPRN overexpression group.
Techniques Used: Over Expression, Expressing, Migration, Transfection, Plasmid Preparation, Wound Healing Assay, Transwell Migration Assay, Negative Control
Figure Legend Snippet: PTPRN expression in normal and lung cancer tissues in TCGA and survival time analyzed by Kaplan–Meier Plotter. (A,B) mRNA expression of PTPRN is analyzed in lung cancer in the TCGA-LUNG database. (C,D) mRNA expression of PTPRN is analyzed in LUAD in the TCGA-LUAD database. (E,F) mRNA expression of PTPRN is analyzed in LUSC in the TCGA database. (G) Kaplan–Meier survival analysis of lung cancer patients indicates that the decrease in PTPRN expression is correlated with favorable prognosis ( P = 0.0057). (H) Kaplan–Meier survival analysis of LUAD patients shows that the decrease in PTPRN expression is correlated with favorable prognosis ( P < 0.0001). (I) Kaplan–Meier survival analysis indicates that there is no correlation between PTPRN expression and prognosis of LUSC patients ( P = 0.96). * P < 0.05, ** P < 0.01. # P > 0.05.
Techniques Used: Expressing
Figure Legend Snippet: Univariate and multivariate analyses of OS in patients with LUAD in TCGA.
Techniques Used:
Figure Legend Snippet: Bioinformatic analysis and western blot indicating that PTPRN expression is related to EMT. (A) The result of GSEA shows that EMT is enriched in the high-PTPRN expression group. PTPRN promotes mRNA expression levels of EMT markers such as (B) N-cadherin, (C) Slug, (D) Snail, (E) Twist, and (F) Vimentin from RNA sequencing data from the TCGA-LUAD database. (G) The PI3K/AKT/mTOR signaling pathway was enriched in the PTPRN high-expression group by GSEA. (H) Left: Expression of EMT markers (Vimentin, N-cadherin, and E-cadherin) is detected in PTPRN-overexpressing A549 and H1299 cells by western blotting. Right: Densitometric analysis of protein expression. (I) Left: Proteins in MAPK/ERK and PI3K/AKT pathways are detected in PTPRN-overexpressing A549 and H1299 cells by western blotting. Right: Densitometric analysis of protein expression. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
Techniques Used: Western Blot, Expressing, RNA Sequencing
Figure Legend Snippet: The association between PTPRN expression and immune response by GSEA in the TCGA-LUAD dataset. GSEA shows that (A) inflammatory response, (B) TNFα signaling via NFκB, (C) TGFβ signaling, (D) negative regulation of immune response, (E) regulation of immune effector process, and (F) regulation production involved in immune response are enriched in high PTPRN expression. (G) PTPRN expression is negatively related to ImmuneScore. (H) There is no correlation between PTPRN expression and StromalScore. (I) PTPRN expression is not related to ESTIMATEScore.
Techniques Used: Expressing
Figure Legend Snippet: Gene set enriched in LUAD samples with high-expression PTPRN.
Techniques Used:
Figure Legend Snippet: PTPRN-related immune infiltration alteration. (A) The fractions of activated memory CD4 T cells, resting NK cells, M0 macrophages, activated mast cells, and neutrophils are higher in the high PTPRN expression group. The proportions of naive B cells, CD8 T cells, resting memory T cells, monocytes, and resting mast cells are lower in high-expression PTPRN. (B) The ratios of different types of immune cells are correlated in the tumor microenvironment.
Techniques Used: Expressing
Figure Legend Snippet: The potential affection of PTPRN on NK cells. PTPRN affects mRNA expression levels of NK cell inhibitory receptors such as (A) LILRB1 and (B) KLRC1 from RNA-sequencing data from the TCGA-LUAD database. (C) Percentages of CD3 – CD56 + cells of NK cells are determined by flow cytometry. (D) NK cytotoxicity assay is conducted with A549 target cells that overexpressed PTPRN on Day 14. The effector-to-target (E: T) ratio is 5:1. A similar assay is conducted with H1299 target cells on Day 14. The E:T ratio is 5:1. NK cells are treated with LUAD cells that overexpressed PTPRN. IFN-γ (E) and TNF-α (F) secretion is analyzed using ELISA. Data are presented as the mean ± SD of three independent experiments performed in triplicate. * P < 0.05, ** P < 0.01.
Techniques Used: Expressing, RNA Sequencing, Flow Cytometry, Cytotoxicity Assay, Enzyme-linked Immunosorbent Assay
Figure Legend Snippet: The effect of PTPRN on EMT and NK cell inactivation in vivo. (A) Images of oe-PTPRN LLC tumor tissues. (B) Average tumor volumes are measured in xenograft mice every 2 days. (C) IHC analysis of vimentin and KLRA1 protein levels in tumor tissues formed from PTPRN-overexpressing cells or control cells. Original magnification, ×400. Scale bars, 50 μm. Data are presented as the mean ± SD of three independent experiments performed in triplicate. * P < 0.05, *** P < 0.001. oe-NC, NC-cDNA group; oe-PTPRN, PTPRN-cDNA group; TGF-β, NC-cDNA with the TGF-β group; and oe-PTPRN+ TGF-β, PTPRN-cDNA with the TGF-β group.
Techniques Used: In Vivo, Control
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